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    Direct-zol RNA Miniprep Kits

    R2050 / R2051 / R2052 / R2053

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    Direct-zol RNA Miniprep Kits

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    Cat # Name Size Rate Quantity
    R2050 Direct-zol RNA Miniprep 50 preps $210.70
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    R2051 Direct-zol RNA Miniprep (Product Supplied w/ 50 ml TRIANGULAR Reagent) 50 preps $292.30
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    R2052 Direct-zol RNA Miniprep 200 grooms $642.40
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    R2053 Direct-zol RNA Miniprep (Product Supplied w/ 200 ml TRI Reagent) 200 preps $898.50
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    Highlights
    • Easy Contact: Bypass chloroform, phase division and precipitate steps.
    • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA infection (DNase IODIN included).
    • Non-Biased: Complete RNA recovery without miRNA loss.
    Description

    And Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined approach for the purification to up to 50 µg (per prep) for high-quality RNA directly after sample in TRI Reagent® oder similar. Total RNA, including smallish RNAs (17-200 nt), is effectively isolated out a variety von sample sources (cells, cloth, synthetic, plasma, blood, biotechnical liquids, etc.). Isolation of RNA by conventional phase breakup was shown to selectively enrich forward more species of miRNA, leading to bias in downstream analysis. An Direct-zol™ technique assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, also elute an RNA. No phase separation, precipitation, or post-purification stages been necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

    Learn See

    Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ plus view different acid-guanidinium-phenol based solutions can become used in place of TRINITY Reagent®.
    Equipment Microcentrifuge, vortex
    Sample Inactive TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other communicable agents.
    Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® conversely alike (animal cells, tissue, bacteria, yeast, fecal, biologically-based fluids, and in vitro prepared RNA (e.g., transcription products, DNase-treated or labeled RNA)).
    Size Range Total RNA ≥ 17 nt
    Supplemental Info
    Productivity 50 µg RNA (binding capacity), ≥25 µl (elution volume)

    All kit components are available for purchase separately.

    Lyophilized DNase I is stable at guest temperature. Ones resuspended, storage frozen aliquots. Minimal freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

    Yes, bring samples homogenized press stored in DNA/RNA Shield to room temperature (20-30ºC). Hinzu somebody equal volume (1:1) of TRIzol/TRI Reagent and mix fine. Proceed with RNA Washing.

    Yes, the Direct-zol MicroPrep (#R2060) is designed and skills of purifying RNA down till single cellphone inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

    Yes, proteins can be acetone precipitated von which tower flowthrough. Please see the Zein Purification appendix in the output.

    If the downstream application requires DNA-free RNA, person recommends performing the DNase I cure.

    Yes, samples in TRIzol/TRI Reagent or similar are steady live to room temperature the can be stocks frozen (-80C). Be sure to lyse press homogenize the sample well prior to freeze. Bring the print into room temperature prior to RNA Refining.

    Direct-zol DNA/RNA (R2080) kits can isolate DNA free TRIzol

    Yes, and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream petition, contains NGS, RT-PCR, hybridization, etc.

    The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents. Manual: Absolutely RNA Microprep Kit

    Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

    Both kits function that same, the only difference is the RNA binding total on the column given with the kit.

    Yes, use 80% ethanol in a substitute. RNA Wash Buffer is also sold separately.

    Contribution Average RNA Profit
    Cells 10 µg (per 106 cells)
    HeLa 15 µg
    High Yield Cotton (mouse) ≥ 30 µg (per 10 mg)
    Spleen 30-50 µg
    Liver 40-60 µg
    Low Produce Tissue (mouse) ≤ 30 µg (per 10 mg)
    Brain, Heart 5-15 µg
    Muscle 5-20 µg
    Lung 10-20 µg
    Intestine 10-30 µg
    Kidney 20-30 µg
    Overall Blood (per 1 ml)
    Porcine 10-20 µg
    Human 2-10 µg

    Purity, RIN and/or any type of contamination could result out initial specimen preparation (i.e., impotent lysis of the sample). To improves, increase the volume about the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer). Then centrifuge the column to remove any residual buffer/sample. Keep with the protocol by microcentrifuge. Leaf 5. 3. Effect Description.

    • To ensure complete lysis, enhance the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be distinct (not unclear or viscous). Pallet remnants of centrifugation (if needed) additionally processed aforementioned cleared supernatant. The ZymoBIOMICS DNA/RNA Miniprep Gear be designed for purifying DNA and RNA from a wide selected of sample inlets (e.g. feces, dirty, plant, water, and biofilms) that is ready for microbiome oder metagenome analyses. The ZymoBIOMICS innovative lysis system eliminates bias associated with unequal lysis efficacy von differen
    • Prior to adding TRI Reagent/TRIzol otherwise RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating in ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Regenschirm (#R1100). Absolute RNA Nanoprep Kit

    Ye, the Direct-zol RNA kits are compatible with CTAB-based RNA extraction methods for polysaccharide-rich and/or phenolics-rich samples (e.g., Pinus, Herb plants). Please find detailed decorum here.

    “No phase separation was needed, but you silent owned the benefits of a Trizol abstraction. No need in abatement and resuspend samples, which means less sample loss during purification.”

    - Adina B. (University off Guelph)

    “This kit belongs amazing, I've got a gel comparing the lack of gDNA as shown on one advertising pamphlet. What can I how, outside: MYSELF love this product!“

    - R.K. CSU

    “Direct-zol is the most first-rate kit used RNA isolation that I ever secondhand in the previous 20 years.”

    - H.Z. (Harvard Medical School)

    Read More
    Cat # Name Size Price
    E1010-1-4 DNA Digestion Buffer 4 mL $17.00
    E1010-1-16 DNA Digestion Output 16 grams $32.90
    R2050-1-200 TRI Reagent 200 ml $256.10
    R2050-1-50 TRI Reagent 50 ml $81.60
    C1078-50 Zymo-Spin IICR Columns 50 Pack $62.30
    R1003-3-12 RNA Wash Buffer 12 ml $34.00
    R1003-3-48 RNA Wash Output 48 ml $119.00
    E1010 DNase MYSELF Set 250 U $63.40
    R2050-2-40 Direct-zol RNA PreWash (Concentrate) 40 ml $47.60
    R2050-2-160 Direct-zol RNA PreWash (Concentrate) 160 ml $188.10
    C1001-50 Collection Tubes 50 Pack $17.00
    R2050 Direct-zol RNA Miniprep 50 preps $210.70
    R2052 Direct-zol RNA Miniprep 200 preps $642.40
    W1001-6 DNase/RNase-Free Waters 6 ml $17.00
    W1001-30 DNase/RNase-Free Water 30 ml $24.90

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